Pair Name	Berbamine, Arcyriaflavin A
Disease Info	[ICD-11: 2A00]	Glioblastoma multiforme		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Phosphorylation	AKT1	hsa207
	Down-regulation	Expression	ALDH1A1	hsa216
	Down-regulation	Phosphorylation	CAMK1G	hsa57172
	Up-regulation	Cleavage	CASP3	hsa836
	Up-regulation	Cleavage	CASP9	hsa842
	Down-regulation	Expression	CCNA2	hsa890
	Down-regulation	Expression	CCNB1	hsa891
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CCNE1	hsa898
	Down-regulation	Expression	CDK1	hsa983
	Down-regulation	Expression	CDK2	hsa1017
	Down-regulation	Expression	CDK4	hsa1019
	Up-regulation	Expression	CDKN1A	hsa1026
	Down-regulation	Expression	ITGA6	hsa3655
	Down-regulation	Phosphorylation	MAPK1	hsa5594
	Down-regulation	Expression	NANOG	hsa79923
	Up-regulation	Cleavage	PARP1	hsa142
	Down-regulation	Expression	POU5F1	hsa5460
	Down-regulation	Expression	PROM1	hsa8842
	Down-regulation	Expression	SOX2	hsa6657
	Down-regulation	Phosphorylation	STAT3	hsa6774
	Up-regulation	Expression	TP53	hsa7157
In Vitro Model	U-87MG ATCC	Glioblastoma	Homo sapiens (Human)	CVCL_0022
	C6	Rat malignant glioma	Rattus norvegicus (Rat)	CVCL_0194
In Vivo Model	U87MG-derived GSCs (2×10⁶ cells/egg) were mixed with ECM gel (40 μL/egg) in the absence or presence of the compounds (5 μg/egg) and placed onto the CAM (8 eggs per group).
Result	Our findings suggest that a novel combination therapy involving berbamine and ArcA could effectively eradicate glioblastoma stem-like cells.